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KMID : 0861020060210040085
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Korea Journal of Herbology
2006 Volume.21 No. 4 p.85 ~ p.92
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Protective effects of added Bo-Yang-Hwan-Oh-Tang on $H_2O_2-induced$ neurotoxicity in SH-SY5Y neuronal cells
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Park Yong-Ki
Han Hyung-Soo
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Abstract
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Objectives : To evaluate the neuroprotective effects of added Bo-Yang-Hwan-Oh-Tang (BHT), we investigated the neuronal death protection effects to oxidative damages in SH-SY5Y neuronal cells.
Methods : To study the cytotoxic effects of BHT on SH-SY5Y cells, the cell viability was determined by MTT assay. To investigate the neuronal death protection of BHT, SH-SY5Y cells were induced oxidative damages by H_2O_2 and then assayed the cell viability and DNA fragmentation. We also investigated DPPH free radical scavenging effect of BHT by tube test.
Results : In MTT assay, 1000{mu}g/ml of BHT was not showed the cytotoxic effect on SH-SY5Y cells. BHT protected SHSY5Y cells from H_2O_2-induced neuronal cell death in a dose-dependent manner. BHT also protected SH-SY5Y cells from H_2O_2-induced DNA fragmentation. BHT effectively scavenged DPPH free radicals in a dose-dependent manner.
Conclusion : These data suggest that BHT may have strong antioxidant effects through the free radical scavenging and neuroprotective effects in human neuronal cells.
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KEYWORD
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antioxidation, apoptosis, Bo-Yang-Hwan-Oh-Tang, H_2O_2-induced neuronal death, neuroprotecitve effect, SH-SY5Y cells
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